To discover sources for novel anti-influenza drugs, we evaluated the antiviral potentialof nine extracts from eight medicinal plants and one mushroom (Avena sativaL.,Hordeum vulgareLinn. var. nudum Hook. f.,Hippophae rhamnoidesLinn.,Lycium ruthenicumMurr.,Nitraria tangutorumBobr.,Nitraria tangutorumBobr. by-products, Potentilla anserinaL.,Cladina rangiferina(L.) Nyl., and Armillaria luteo-virens) from the Qinghai–Tibetan plateau against the influenza A/H3N2 virus. Concentrations lower than 125μg/mL of all extracts demonstrated no significant toxicity in MDCK cells. During screening, seven extracts (A. sativa, H. vulgare, H. rhamnoides, L. ruthenicum, N. tangutorum, C. rangiferina, and A. luteo-virens) exhibited antiviral activity, especially the water-soluble polysaccha-ride from the fruit body of the mushroom  A. luteo-virens. These extracts significantly reduced the infectivity of the human influenza A/H3N2 virus invitro when used at concentrations of15.6–125μg/mL.Two extracts (N. tangutorumby-products and P. anserina) had no A/H3N2 virus inhibitory activity. Notably, the extract obtained from the fruits of N. tangutorumand N. tangutorumby-products exhibited different anti-influenza effects. The results suggest that extracts of A. sativa, H. vulgare, H. rhamnoides, L. ruthenicum, N. tangutorum, C. rangiferina, and A. luteo-virens contain substances with antiviral activity, and may be promising sources of new antiviral drugs.